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簡(jiǎn)體中文|
English| Launch：2023-09-14 |
Sulfonamides refer to a class of drugs with p-aminobenzenesulfonamide structure, which are commonly used broad-spectrum antimicrobials. Sulfonamides are widely used in the prevention and treatment of bacterial infectious diseases in animal breeding because of their wide antibacterial spectrum, high efficiency, low toxicity and low price. However, due to the long time of action and metabolism of sulfonamides in animals, improper use will lead to excessive sulfonamides residues in animal edible tissues and long-term consumption of foods containing excessive sulfonamides residues. It may cause the accumulation of sulfonamides in the body and threaten human health. May cause allergic reactions in the human body, damage to the immune system and blood system, damage to the urinary system and teratogenic, mutagenic and carcinogenic and other adverse effects.
At present, the main detection methods of sulfonamide include enzyme-linked immunosorbent assay, liquid chromatography-mass spectrometry and so on. Enzyme-linked immunosorbent assay (Elisa) has been widely used as a screening method, but it is easy to appear false positive. Although liquid chromatography and liquid chromatography-mass spectrometry can obtain more accurate results, most of them have a series of problems, such as expensive instruments and equipment, complex sample treatment methods, long detection time and so on, which can not meet the current situation of frequent food safety problems. sample detection urgently needed for real-time, on-the-spot, effectiveness requirements. Therefore, it is of great significance to develop a fast, convenient and sensitive method.
Based on the unique advantages of high sensitivity and simple pretreatment of Raman spectroscopy, such as sea optoelectronics developed a kit for the detection of sulfonamides in food of animal origin by surface enhanced Raman spectroscopy. Let's go and have a look.
Application case
1. Experimental object
Detection substrate: milk.
Detection items: sulfa mixed standard, sulfonamide m-dimethoxine.
Note: milk has been tested by liquid chromatography-mass spectrometry to confirm that it does not contain sulfonamides.
2. Instruments and consumables
3. Experimental scheme
Before the beginning of this experiment, the milk was detected by liquid phase-mass spectrometry to confirm that there were no sulfonamides residues in the milk samples. Carry on the positive standard treatment to the experimental sample after the test, and the specific information of the sample is shown in the table below.
4. Testing method
The experimental scheme and the reagents used in this test are provided by Raman rapid detection kit for oceanhood sulfonamides antibiotics, and milk pre-processing and spectrum collection are completed by oceanhood self-developed all-in-one machine and box-type Raman spectrometer, respectively. Then the data are identified by intelligence test E+ software. For the detailed testing process of the sample, please refer to the manual of Raman rapid detection kit for oceanhood sulfonamides antibiotics.
5. Test result
A total of five tests were carried out in this experiment. in the first group, the milk samples without any sulfonamides were tested, and the milk was treated according to the instructions of the Raman rapid detection kit for sulfonamides. The spectrum was determined by the intelligent detection E+ software, no Raman characteristic shift of sulfonamides was found, indicating that no sulfonamides were detected in the milk samples.
In experiment 2, 3 and 4, different concentrations of sulfamethoxine were added to the milk matrix, and the added concentrations were 0.1,0.2,0.5 respectively (the experimental test spectrum is as follows). By comparing the instructions of the kit and intelligence test E +, the Raman characteristic shifts of 664 and 822cm-1 sulfonamides were found in the three groups of experiments. The Raman characteristic shift intensity of the two sites was positively correlated with the concentration of sulfamethoxine, which indicated that sulfamethoxine added in milk had been detected. In the last group of experiments, 12 kinds of sulfonamides were added to milk samples, and the detection results were similar to those of milk samples with sulfamethoxine. Obvious Raman characteristic shifts (664, 822cm-1) of sulfonamides were observed in the test spectra, indicating that 12 sulfonamides could also be detected. The specific experimental results are shown in the table below.
6. Conclusion
Oceanhood self-developed sulfonamides rapid detection kit combined with pretreatment box and box Raman spectrometer can be used to detect sulfonamides in milk. 0.1mg/kg sulfonamides mixed standard and single sulfonamides can be detected.
